EN 15829:2010 – Foodstuffs – Determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit and dried figs – HPLC method with immunoaffinity column cleanup and fluorescence detection

1 Scope
This European Standard specifies a method for the determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit and dried figs by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 1,1 µg/kg to 11 µg/kg.
For further information on the validation, see Clause 9 and Annex B. WARNING — The use of this standard can involve hazardous materials, operations and equipment. This standard does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 3696:1995, Water for analytical laboratory use ― Specification and test methods (ISO 3696:1987) 3 Principle A test portion is extracted with a mixture of methanol and phosphoric acid. The extract is filtered, diluted with phosphate buffered saline, and applied to an immunoaffinity column containing antibodies specific for ochratoxin A. The ochratoxin A is isolated, purified and concentrated on the column then released with elution solvent. Ochratoxin A is quantified by reverse-phase high performance liquid chromatography (HPLC) with fluorescence detection.
4 Reagents
4.1 General Use only reagents of recognized analytical grade and water complying with grade 1 of EN ISO 3696:1995, unless otherwise specified. Solvents shall be of quality for HPLC analysis. Commercially available solutions with equivalent properties to those listed may be used. WARNING — Dispose of waste solvents according to applicable environmental rules and regulations. Decontamination procedures for laboratory wastes have been reported by the International Agency for Research on Cancer (IARC), see [1].
4.2 Helium purified compressed gas
4.3 Disodium hydrogen phosphate, anhydrous or Na 2 HPO 4 ·12 H 2 O
4.4 Potassium chloride
4.5 Potassium dihydrogen phosphate
4.6 Sodium chloride
EN 15829:2010 – Foodstuffs – Determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit and dried figs – HPLC method with immunoaffinity column cleanup and fluorescence detection