EN 15550:2007 – Animal feeding stuffs – Determination of cadmium and lead by graphite furnace atomic absorption spectrometry (GF-AAS) after pressure digestion
1 Scope
This European Standard specifies a method for the determination of the elements cadmium and lead in animal feeding stuffs by graphite furnace atomic absorption spectrometry (GF-AAS) after pressure digestion.
The method limit of quantification for each element is dependent on the sample matrix as well as the instrument. For cadmium a limit of quantification of 0,05 mg/kg should normally be obtained while for lead, a limit of quantification of 0,5 mg/kg should be obtained.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
ISO 6498, Animal feeding stuffs – Preparation of test samples
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
limit of detection (LOD)
smallest measured content from which it is possible to deduce the presence of the analyte with reasonable statistical certainty
NOTE The limit of detection is numerically equal to three times the standard deviation of the mean of blank determinations (n ≥ 10, where n = number of measures) performed under reproducibility conditions.
3.2
limit of quantification (LOQ)
lowest content of the analyte that can be measured with reasonable statistical certainty
NOTE If both trueness and precision are constant over a concentration range around the limit of detection, then the limit of quantification is numerically equal to ten times the standard deviation of the mean of blank determinations (n ≥ 10,were n = number of measures) performed under reproducibility conditions.
3.3
feed additives
substances that comply with the definition of feed additives given in the Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition
3.4
animal feeding stuffs
substances that comply with the definition of animal feeding stuffs given in the Regulation (EC) No 178/2002
EN 15550:2007 – Animal feeding stuffs – Determination of cadmium and lead by graphite furnace atomic absorption spectrometry (GF-AAS) after pressure digestion