BS EN 1657:2000 – Chemical disinfectants and antiseptics Ð Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in veterinary field Ð Test method and requirements (phase 2/step 1)

1 Scope This European Standard specifies a test method (phase 2/step 1) and the minimum requirements for fungicidal activity of chemical disinfectant and ant iseptic products that form a homogeneous physically stable preparation in standardized hard water. This European Standard is appl icable to products for use in the veterinary field, i.e. in the . breeding, husbandry, product ion, transport and disposal of all animals except when in the food chain following . death and entry to the process ing industry.
NOTE 1 Using this European Standard it is not possible to determine the fungicidal activity of an undilted product as some dilution is always produced by adding the inoculum and interfering substance. Produets can only be tested at a concentration of 80 % or less. NOTE2 This method corresponds to a phase 2 step 1 test (see annex F).
2 Normative references
This European Standard incorporates by dated or undated reference, provisions from other publ icat ions. These normative references are cited at the appropriate places in the text and the publicat ions are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publ ications apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publicat ion referred to applies. EN 1040: 1997, Chemical disinfectants and antiseptics – Basic bactericidal activity – Test method and requirements (phase 1).
EN 1275: 1997，Chemical disinfectants and antiseptics – Basic fungicidal activity – Test method and requirements . (phase 1).
EN 12353, Chemical disinfectants and antiseptics – Preservation of microbial strains used for the determination of bactericidal and fungicidal activity. IS0 4793, Laboratory sintered (fitted) flters . Porosity grading – Classification and designation.
3 Terms and definitions
For the purposes of this standard, the fol lowing terms and definitions apply:
3.1
product (for chemical disinfection and/or antisepsis)
chemical agent or formulat ion used as a chemi cal disinfectant or antiseptic [EN 1040: 1997]
3.2
fungicide
product which kills fungi including their spores under defined condit ions [EN 1275: 1997]
NOTE
The adjective derived from “fungicide” is ‘fungi cidal’.
3.3
fungicidal activity
capability of a product to produce a reduction in the number of viable vegetative yeast cells and mould spores of re levant organisms under condit ions defined by this European Standard
4 Requirements
The product diluted in standardized hard water when tested, in accordance with clause 5, shall demonstrate at .
1east a 10* logari thmic reduct ion in viable counts when the tes t organi sms are vegetat ive cells of Candida albicans and the spores of Aserilus niger. The test is carried out under simulated low (3, 0 g/1 bovine albumin) or high soiling conditions (10 g/1 yeast extract and 10 g/1 bovine albumin) according to its practical appl icat ions and under the requi red test conditions (10° C (30° C for teat disinfectants) 30 min, 2 referenced strains). Where appropriate additional and optional contact times of 1 min士5 s，5min士10s and 60min士10s and additional and optional temperaturesof4″ C, 20。Cand40。C are specified.
5. Test method
5.1 Principle
5.1.1 A test suspension of yeast cells and mould spores in a solution of interfer ing substances is added to a prepared sample of the product under test. The mixture is maintained at 10。c士1°C (or 30°C士1° C for teat disinfectants). At a contact time of 30 min士10 s an aliquot is taken; the fungicidal action in this portion is immediately neutralized or suppressed by a val idated method.
The method of choice is dilution-neutralization. If a sui table neutralizer cannot be found, membrane filtration is used. The number of surviving yeast cells or mould spores in each sample is determined and the reduction in viable counts calculated. Preliminary tests leading to the choice of the inactivat ion me thod should be carried out before the actual test, for each fungal strain.
However, after choosing the method (di lution- neutralization or membrane filtration)， it is necessary to check the inactivation of the carry over in parallel with the actual test.
BS EN 1657:2000 – Chemical disinfectants and antiseptics Ð Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in veterinary field Ð Test method and requirements (phase 2/step 1)