BS EN ISO 10273:2003 – Microbiology of food and animal feeding stuffs — Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica

1 Scope
This International Standard specifies a horizontal method for the detection of Yersinia enterocolitica presumed to be pathogenic to human subjects. This International Standard is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 6887 (all parts), Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination
ISO 721 8, Microbiology of food and animal feeding stuffs — General rules for microbiological examinations,and Amd.1 :2001
ISO 8261 , Milk and milk products — General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination
ISO/TS 1 1 1 33-1 , Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
presumptive pathogenic Yersinia enterocolitica
psychrotrophic bacteria forming characteristic colonies on solid selective media and having the biochemical properties meeting the pathogenicity criteria described when the test is carried out in accordance with this International Standard
4 Principle
4.1 General
Presumptive pathogenic Yersinia enterocolitica are detected by the following three successive stages.
4.2 Enrichment in selective liquid media
The test portion is inoculated into two enrichment media
peptone, sorbitol and bile salts (PSB) broth, and
irgasan , ticarcillin and potassium chlorate (ITC) broth.
The ITC broth is incubated at 25 °C for 48 h and the PSB broth for 3 to 5 days.
BS EN ISO 10273:2003 – Microbiology of food and animal feeding stuffs — Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica